khtT [2017-8-11 16:0:3]

K /H antiporter for K efflux

locus: BSU09860

pI: 4.89

mw: 18.59 kDa

protein length: 165 aa

gene length: 495 bp

function: potassium ion efflux

product: K /H antiporter for K efflux

essential: no

synonyms: yhaT

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,060,427 → 1,060,924

The protein

[SW|Domains]

• contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score x])

[SW|Localization]

• cell membrane (peripheral) (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]
• GP2078 (''[gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

Expression vector

• pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 27935846

Original publications

• 14987767,17679694,24330391